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Coding

Part:BBa_K771107

Designed by: Guo Huaqing   Group: iGEM12_SJTU-BioX-Shanghai   (2012-09-24)

SH3domain

Components of interacting proteins

Usage and Biology

12SJTU SH3dl.gif

The SH3 domain along with its ligand(Part:BBa_K771108) comes from Mus musculus, which could dimerize spontaneously. The original dimer was introduced by Dueber et al., 2012. This pair of dimer is a perfect material to construct the dimerizable proteins. In Membrane Magic system designed by [http://2012.igem.org/Team:SJTU-BioX-Shanghai 2012 SJTU-BioX-Shanghai Team], the SH3 Domain is located in Membrane Anchor 2, while the SH3 ligand on Membrane Anchor 3. A flexible linker3(FL3) is added to the N-terminus of the domain, enabling its proper interaction with its domain on Membrane Anchor 3.

Related Parts

Part:BBa_K771108, SH3 ligand


Part:BBa_K771002, Membrane Anchor 2, containing SH3 domain


Part:BBa_K771003, Membrane Anchor 3, containing SH3 ligand

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 145


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Categories
Parameters
n/aSH3domain